Catalog #WM1000

YesBlot™ Western Marker I (10-200 kDa)
250µl unit, 5µl per well (50 applications).

Protein Ladder designed for Western Blot  
• Easy to identify bands
• 10dKA-200 kDA
• 10 bands for Antibody (Ab) detection
• 4 predestined bands

Description and Information 
SMOBIO's YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer. YesBlot™ Western Marker I, a protein ladder for Western Blots, performs dual functions. FIRST, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. SECOND, ten IgGbinding proteins can be immuno-detected on film or by CCD imaging. YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
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Storage:  4°C ≥ 3 months; -20°C ≥ 24 months.

Usage Recommendation: 1.5~2.5 μl per load (lane) for two-step Western blot using 1st Antibody (Ab) followed with 2nd Ab conjugated with reporter enzymes.  Use 2.5~5 μl per load for one-step Western blot using 1st Ab conjugated with reporter enzymes. Apply for thicker (> 1.5 mm) or larger gel.

Contents
YesBlot™ Western Marker I contains recombinant IgG binding proteins, Glycerol, SDS, and tracking dyes in a Tris-HCl buffer. 

Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control
Under suggested conditions, PM1500 ExcelBand All Blue Regular Range Protein Marker resolves 10 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane. 

Note this Information
This product is a restricted product and can only be purchased by approved account holders. 
For Research Use Only. Not for use in diagnostic procedures.

Note, Additional Shipping Fees apply.
Overnight Shipping Fees and Dry Ice Fees.
MSDS is shipped with product.