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TBS Buffer (10X)TBS (Tris Buffered Saline), the most commonly used wash and incubation buffer for Western blotting, is in a convenient 10X concentrate. Ultra pure reagents and stringent quality control ensures the best results from your experiments. When diluted to 1X, National Diagnostics TBS yields a buffer containing 25mM tris, 137mM sodium chloride and 2.7mM potassium chloride.