Magnetic Bead Purification Technology
The Mag-BIND® FFPE RNA 96 kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. The sample is first processed in a special buffer by heating to deactivated RNases/DNase and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then mixed with Mag-BIND® particles to capture the capture RNA. After a few quick wash steps, purified RNA can be eluted with water or low salt buffer.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|
| Downstream Application | PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification |
| Elution Volume | 30-50 µL |
| Starting Material | FFPE tissue |
| Starting Amount | 2-5 paraffin sample sections between 5-10 μm |
| Processing Mode | Automated; Manual |
| Throughput | Up to 96 |
| RNA Binding Technology | Magnetic Beads |