The E-Z 96® HP Total RNA Isolation kit combines the reversible binding properties of HiBind® RNA technology with a specially designed buffer system which can effectively remove DNA before RNA isolation. Samples are first lysed and homogenized in a specially designed denaturing buffer (GTC), which immediately inhibits the activity of RNase and DNase. The lysate is then passed through a E-Z 96® DNA Clearance Plate which will trap the genomic DNA. The flow-through lysate is then ran through a E-Z 96® RNA Plate to bind RNA. After two wash steps, purified RNA is eluted with RNase-free water.