Based on the same principle of E.Z.N.A.® Total RNA Kit II, the Ultra-Pure Total RNA Midi Kit is designed for the isolation of total RNA from 1 x 10^8 cultured eukaryotic cells or 500 mg of all type of animal and plant tissues. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue samples including difficult-to-lyse fibrous and fatty tissues such as skeletal muscle, heart, brain, adipose tissues and plant, fungal samples. Compared to other standard silica-column procedure, E.Z.N.A.® Ultra-Pure Total RNA Midi Kit provide higher yield and better quality of RNA from all types of tissues. RNA purified using the E.Z.N.A.® Ultra-Pure Total RNA Midi method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation. |
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