The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit offers a versatile solution for the isolation of high-quality DNA from a wide variety of samples, including fresh or frozen cultured cells and tissues, buccal swabs, mouse tail snips, dried blood spots, up to 250 µL whole blood, and up to 500 µL saliva.

Our cutting-edge Mag-Bind® technology reduces overall processing time by providing a quick magnetic response. This Kit combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with our proprietary chemistries to provide a rapid and reliable method for DNA isolation from a variety of samples. Purified DNA is high-quality and suitable for use in myriad downstream applications, including PCR, NGS, and enzymatic reactions.

Ready-to-implement protocols are available for the following platforms:

Accuris IsoPURE™ 96
Hamilton Microlab® STAR
Hamilton Microlab® NIMBUS
Tecan Fluent®
Tecan Freedom EVO®
Thermo Fisher Scientific KingFisher® Apex
Thermo Fisher Scientific KingFisher® Flex

FEATURES	SPECIFICATIONS
Starting Amount	100-250 μL blood samples, saliva, swabs, mouse tails, dried blood spots or 5 x 10⁶ cultured cells.
Elution Volume	50-200 µL
Technology	Magnetic Beads
Processing Mode	Automated, Manual
Throughput	8 – 384
Note	Downstream applications: NGS, qPCR, microarray

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Materials and Reagents that need to be Supplied by the End-User:
• 100% ethanol
• 100% isopropanol
• Nuclease-free water
• Magnetic separation device (Recommend AlpAqua Magnum™ EX, Part#A000380)
• Vortexer
• Heat block, incubator, or water bath capable of 70°C
• 96-well Microplate (500 μL) (Cat# EZ9604) or desired elution plate
• 2 mL 96-well deep-well plates (Recommend Nunc, Part#278752) or
desired plate compatible with the magnetic separation device
• Multichannel pipettes and reagent reservoirs
• Sealing film (Cat# AC1200)
• Optional: RNase A (10 mg/mL)
• Optional: PBS

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Yield from Whole Blood

Figure 1. DNA yield from 200 µL whole blood extracted using the Mag-Bind® Blood & Tissue DNA HDQ 96 Kit on the ABI MagMAX 96 instrument. DNA yield was determined by PicoGreen® quantification.

Yield from Buccal Swabs

Figure 2. Genomic DNA was extracted from buccal swab samples using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit and Company A’s genomic DNA extraction kit. 10% of DNA extracted from each sample was analyzed on a 0.8% agarose gel.

High Quality, Inhibitor-free

Figure 3. Genomic DNA was extracted from 1,500 µL whole blood using Omega Bio-tek’s Mag-Bind® Blood & Tissue DNA HDQ 96 Kit. The elution volume was 500 µL. DNA was analyzed on Thermo Scientific’s NanoDrop® 2000c. 50 ng of DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.

DNA Size

Figure 4. The sizes of genomic DNA purified using Mag-Bind Blood & Tissue DNA HDQ Kit were determined by electrophoresis. Sample 1 and 2 were gDNA from human blood. L is 50 kb genomic ladder for Agilent TapeStation 2200. The size of gDNA is around 60 kb.

Omega Biotek Mag-Bind Blood & Tissue DNA HDQ 96 Kit, 24 x 96 preps

Tissue, Blood & Cultured Cell Kit
Mag-Bind Blood & Tissue DNA HDQ 96 Kit, 24x96 preps (2304)
• DNA Extraction from Blood, Saliva, Buffy Coat, Buccal Swabs, Cultured Cells, and Tissues
• Automation Friendly Formats allows for up to 384 samples to be processed in 2.5 hours
• High Quality DNA is ready for NGS, Microarrays, and qPCR
• CE-IVD marked kit is available

(Description below. Register for discount.)

SKU: M6399-02

Old price: $5,120.40

Price: $4,269.00

Qty:

Yield from Whole Blood

Yield from Buccal Swabs

High Quality, Inhibitor-free

DNA Size

Omega Biotek Mag-Bind Blood & Tissue DNA HDQ 96 Kit, 24 x 96 preps

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