Overview
The E.Z.N.A.® Blood DNA Kit provides rapid total DNA isolation from 1-250 µL of fresh and frozen anticoagulated whole blood. The E.Z.N.A.® Blood DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® Blood DNA method is ready for applications such as PCR, Southern blotting, or restriction enzyme digestion.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|
| Starting Amount | 1-250 µL |
| Starting Material | Fresh and frozen anticoagulated whole blood, body fluid, buccal swab, dried blood, buffy coat |
| Yield | 100 µg |
| Elution Volume | 100-200 μL |
| Technology | HiBind® DNA Mini Column |
| Processing Mode | Manual, centrifugation/vacuum |
| Throughput | 1-24 |
E.Z.N.A.® Blood DNA Kit purified high quality genomic DNA from saliva sample stored in Oragene tubes.
Figure 1. Genomic DNA isolated using the E.Z.N.A.® Blood DNA Mini Kit. DNA was purified from 4 mL saliva stored in Oragene® tubes using the E.Z.N.A.® Blood DNA Mini Kit. DNA was eluted in 500 µL and quantitated using the Thermo Scientific NanoDrop® 2000 spectrometer.
Genomic DNA yields from 1 mL human whole blood by E.Z.N.A.® Blood DNA Kit
Table 1. Yield of genomic DNA purified using the E.Z.N.A.® Blood DNA Mini Kit. DNA was purified from packed blood cells of 1 mL whole blood using the E.Z.N.A.® Blood DNA Mini Kit. DNA was eluted in 500 µL and quantitated using the Thermo Scientific NanoDrop® 2000.