Accuris, One-Step RT-PCR Kits

Accuris - One-Step RT-PCR Kits
'Latest Generation' kits, features convenient cDNA Synthesis and PCR in a Single Tube for end-point analysis - from 1pg total RNA.

• Formulated for Highly Specific and sensitive RT-PCR, from any RNA templates • Detects low copy transcript
• 2X master mix with Accuris Hot Start Taq Polymerase for improved specificity
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature
• Compatible with ALL Real Time thermal cyclers

Our thermostable, MMLV-derived Reverse Transcriptase is coupled with Accuris Hot Start Taq and RNase inhibitor for highly sensitive assays.

Click HERE - for PCR Taq Product Selector Guide
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One-Step kits for Pathogen Detection and Accurate Gene Expression Analysis

The Accuris One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and Accuris Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The Accuris One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.