Click HERE - for PCR Taq Product Selector Guide
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Our Accuris qMAX cDNA Synthesis Kits are superior kits for processing cDNA (complimentary DNA) from mRNA. Because cDNA is more stable than mRNA, our kit is ideal for gene expression, gene cloning, and making cDNA libraries.
cDNA is a vital research tool for Molecular Biologists. qMax DNA Synthesis Kits from Accuris provides a quick, accurate and reproducible way to obtain high quality cDNA of total RNA or mRNA.
- Rapidly generate high quality, qPCR ready cDNA.
- Unbiased, complete sequence representation.
- Thermostable qMax RT with RNase inhibitor.
- Optimized for use across a wide dynamic range.
- Two tube format for easy reaction set up
cDNA is a vital research tool for Molecular Biologists. The centerpiece of the qMax cDNA Synthesis Kits is qMax RT – a thermostable reverse transcriptase that allows reactions to be carried out at higher temperatures, thus eliminating the problems created by secondary structure in the RNA. A potent RNase inhibitor maintains integrity of the starting material. Unbiased representation and synthesis are ensured by a precise mix of anchored oligo (dT) and hexamer primers in the 5X buffer system. Buffer and enzyme are supplied in separate tubes to allow for negative control reactions. Accuris qMax qPCR mixes are the ideal partner for quantitative PCR of cDNA.
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Our original cDNA Synthesis Kit is a 2-tube format for easy reaction set up and is ideal for 4pg to 0.5µg of input RNA. One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a nonbiased population of cDNA. Download our cDNA Synthesis protocol for full details.
Our 1st Strand cDNA Synthesis Flex Kit includes four components: a high capacity Reverse Transcriptase, an optimized 5X buffer, and separate solutions of oligo (dT) primers, and random hexamer primers. This multi-component format is ideal for 10pg to 2.0µg of input RNA and allows for greater flexibility in assay design.
Download our First Strand cDNA Synthesis Flex Kit protocol for full details.
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| Item No | Description | Reactions (20µl volume) |
|---|
| PR2100-C-S | Accuris qMax cDNA Synthesis Kit, sample | 5 |
| PR2100-C-25 | Accuris qMax cDNA Synthesis Kit | 25 |
| PR2100-C-100 | Accuris qMax cDNA Synthesis Kit | 100 |
| PR 2100-C-250 | Accuris qMax cDNA Synthesis Kit | 250 |
| PR2110-S | Accuris qMAX First Strand cDNA Synthesis Flex Kit, sample | 5 |
| PR2110-50 | Accuris qMAX First Strand cDNA Synthesis Flex Kit, 50 reactions | 50 |
| PR2110-200 | Accuris qMAX First Strand cDNA Synthesis Flex Kit, 200 reactions | 200 |
