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Mini sized Bacteria RNA EZ-SpinKit (Homogenizer Columns included)
- Compare with QIAGEN’s RNeasy® #74104, #74106 -

Efficient Isolation of RNA from Bacteria

Bacterial Total RNA Isolation Mini-SpinKits are specially designed for isolating total cellular RNA from Gram-positive and Gram-negative bacteria. These kits use a lysis method that is custom designed for difficult bacteria in conjunction with our proprietary silica-based High-Bind EZ-SpinKit columns to produce reliable RNA isolations in less than 20 minutes. Average yield from bacteria (1x10⁹) is 10-30μg. Purified RNA can be used in any downstream applications without the need for further purification.

RNA Isolation and Purification

Manipulation of RNA is inherently more challenging than that of DNA since RNase is not only ubiquitous to most laboratories, it is also extremely unstable. Omega RNA EZ-Kits and EZ-96 plate Kits allow for reliable isolation of RNA from a variety of sources with its HighBind spin column method. These systems involve the simultaneous lysis of cells from a variety of sources of endogenous RNases. This is accomplished by using proprietary denaturing buffers that enhance the reversible binding of RNA to the HighBind silica matrix. While cells can be lysed directly, tissues are disrupted or homogenized using common laboratory supplies. Binding conditions are further adjusted and lysates are applied to the spin columns that selectively bind RNA molecules greater than 200 nt in length, thus favoring mRNA isolation. Successive washes with specially formulated buffers remove inhibitors and contaminants, allowing subsequent elution of pure RNA with water. As with other Omega products, phenol and chloroform extraction are eliminated. Kits are tailor made for specific cells and tissue sources to ensure consistent and reliable RNA preps, every time.

Storage and Stability - LSPI’s Omega RNA EZ-SpinKit and EZ-96 plate Kit isolation components are guaranteed for at least 12 months from the date of purchase, when stored as recommended in supplied instruction manuals.

Three Column Designs for Mini EZ-SpinKits

1.) Q-Spin Columns, our original spin column designed for centrifugation protocols, feature a large capacity reservoir above the HighBind DNA resin and a flat bottom. This allows for a larger volume of sample to be processed in the column at one time with better flow-through during centrifugation. Expect higher yields with Q-spins.

2.) V-Spin Columns are equipped with a standard outlet luer at its bottom for an easy to use and leak-free connection to vacuum manifolds or luer hub needles. V-Spin Columns can be used with centrifugation and/or vacuum manifold protocols.

3.) S-Spin Columns are designed like Q-Spins, includes an attached cap.