Agarose from Benchmark Scientific

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Benchmark Agarose LE
Benchmark Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact.  Agarose LE can be used for analyses of nucleic acids and proteins of various sizes (150 bp to 25 kbp). Its low EEO (≤ 0.13-mr ) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times.  This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix.  The consistently low EEO also provides a reduction in band distortion (caused by counter flow) that can result from the presence of excessive sulfate-rich negative ions.  Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio. Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added. Available in 25g, 100g and 500g quantities.
Benchmark Agarose LE in EZ PACK™ Agarose Tablets
The quick dissolving EZ Pack tablet contains 0.5g (500mg) of Agarose LE, eliminating the hands-on time and inaccuracies normally associated with weighing. Use of the tablet is not only convenient and cleaner, it also provides better consistency andreproducibility gel to gel. Gel preparation is simple – add the desired number of tablets to electrophoresis buffer, allow to sit for two minutes and then heat and pour as usual. The resulting gels are highly transparent, have exceptional thermal stability (ensuring safe and easy handling) and exhibit exceptionally low absorption of chemical staining agents. EZ Pack Agarose Tablets are supplied in convenient blister packs for safe, clean dispensing.
See EZ Chart Tablet Photo (above) for Buffer Volumes required to achieve desired gel strengths.

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Specialty Agaroses
•  Benchmark’s Agarose LM is a low melting point agarose ideal for preparative gels. The melting and gelling points are well below that of the DNA/RNA denaturation temperatures, allowing for recovery of nucleotides from the gel. Also useful for cloning of tissue culture cells and viral plaque assays.
•  Benchmark’s Agarose 3:1 is blended high resolution agarose is designed for separation of fragments less than 1,000bp. Gels made with Agarose 3:1 are suitable for analytical and blotting applications.
•  Benchmark’s Agarose HR is a high resolution agarose is designed for separation of fragments less than 20-800bp. Gels made with Agarose HR are suitable for high resolution of PCR products and analysis of small fragments such as STRs and AFLPs.
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All Benchmark agarose is manufactured under a “green” process that eliminates the use of organic solvents. They provide gels with enhanced resolution and clarity, low EEO and low background. Tested to be RNase, DNase, Protease and Endonuclease free. Supplied in 100g bottles.