DM1200
AccuBand™ 50 bp DNA Ladder
500 μl (0.5ml), 100 loadings
Description
The DM1200 AccuBand 50 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DM1100 DNA marker is composed of 13 individual DNA fragments: 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100, and 50 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (500 bp and 200 bp) for easy referencing. In addition, the low range Orange G tracking dye mimics the migration of a 50 bp dsDNA during electrophoresis is also added for real time monitoring.
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
50 ~ 1,500 bp
Concentration
54 µg/ 500 µl
Recommended loading volume
5 µl/ well
Description
AccuBand 50 bp DNA marker II is composed of 13 individual DNA fragments, presenting 2k, 1k, 750, 500, 250, 100, and 50bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBandTM 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBandTM 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis.
Buffering
10 mM Tris-HCl (pH 8.0), 10 mM Na-EDTA, 0.025% Orange G, 0.005% Xylene cyanol FF and 10% Glycerol.
Quality Control
• 6 defined bands are observed during electrophoresis in agarose gel
• No nuclease activity is detected by a direct nuclease activity assay.
Storage
Protected from light
Room temperature ≥ 6 months
4°C ≥ 12 months
-20°C ≥ 36 months
Note this Information
This product is a restricted product and can only be purchased by approved account holders.
For Research Use Only. Not for use in diagnostic procedures. .
Shipping Fees apply.