E-Z 384 Plant DNA HT Kit
This kit enables rapid and high–throughput isolation of genomic DNA from a wide variety of plant tissue samples. Up to 768 fresh, frozen, or dried plant tissue samples rich in polyphenols, polysaccharides or having a lower DNA content can be processed in approximately 1.5 hours, when processing two plates in parallel. The system combines the reversible nucleic acid-binding properties of our HiBind® silica matrix with the speed and versatility of a 384-well format to maximize sample processing throughput. The purification procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with a unique binding system to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue samples. The system eliminates the need for chloroform extractions traditionally associated with CTAB- based lysis methods. The eluted DNA is inhibitor-free and ready for downstream applications such as genotyping, plant breeding, population level analyses, Next Generation Sequencing, PCR, and more.
Features and Benefits
- Productivity – Allows processing of up to 768 samples in parallel
- Robust – Reliable results from a variety of sample types
- Safe – No organic extractions
- Performance – High-quality DNA sufficient for most applications
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|
| Starting Material | Fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a low DNA content |
| Starting Amount | 30 mg fresh/10 mg dry plant sample per well |
| Elution Volume | 65 µL |
| Processing Mode | Manual (Centrifugation or Vacuum) |
| Throughput | 1 - 768 (process two 384-plates in parallel) |
| Nucleic Acid Binding Technology | 384-well silica filter plate |
| Processing time | 1.5 h |
| Downstream Application(s) | Genotyping, plant breeding, population level analyses, next generation sequencing, PCR, and more. |
| Storage Conditions | |
Product Data
Average DNA Yield from Plant Samples
Table 1. The average DNA yield from 2 leaf punches of each sample type extracted using the E-Z 384 Plant DNA HT Kit and eluted in
65 μL volume.The DNA yield was determined using Promega’s QuantiFluor® dsDNA system.
Average Ct Values Comparison from Different Plant Types
Figure 1. Average Ct values obtained amplifying the purified DNA from various plant samples using the E-Z 384 Plant DNA HT Kit. The ΔCt values were ~3.3, indicating high-quality DNA that is free of inhibitors.
Extraction Method Comparison
DNA Yield from Canola Leaves
Average Ct Values from Canola Leaves
Figure 2. The average DNA yield from 2 leaf punches of canola leaves (n=10) extracted following centrifugation and vacuum protocols of the E-Z 384 Plant DNA HT Kit is shown on the left. The purified DNA is eluted in 65 ul volume and DNA yield was determined using Promega’s QuantiFluor®dsDNA System. The average Ct values were obtained amplifying the purified DNA (right). The ΔCt values were ~3.5, indicating high-quality DNA that is free of inhibitors.