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Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. Up to 5 mL of overnight  cultures can be used for high copy number plasmids and 10 mL for low copy number.

The E.Z.N.A.® Endo-Free Plasmid Mini Kit I integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0.1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

  • Speed – 30 minutes or less
  • Safety – No Phenol/chloroform extractions
  • Versatility – Spin and vacuum formats available
  • Quality - Endotoxins efficiently reduced (<0.1 EU/µg)

Omega Biotek Endotoxin-Free, Plasmid DNA Mini Kit I, 5 preps

Endo-Free, Plasmid DNA Mini Kit, 5 preps (Description below)
SKU: D6948-00
Old price: $24.30
$23.15
Products specifications
Attribute nameAttribute value
Sample Size1-15mL
Binding Capacity35 μg
Processing Time35 min
Plasmid TypeHigh & Low Copy Plasmids
Starting Amounts1-5 mL
Restriction Enzyme, Sequencing, PCR, TransformationYes
Sensitive Cell Line & Primary Cell TransfectionNo
Transfection, In vitro transcriptionNo
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