Applications:

• Western Blotting, Immunoblotting, Southern and Northern Transfers, Nucleic Acid Dot/Slot Blotting, Colony Hybridizations and Plaque Lifts, and Expression Screening

NitroBind is the membrane of choice for all protein or immuno-blotting applications. NitroBind does not contain cellulose acetate, a low binding polymer found in competitive brands. This assures the highest binding and sensitivity (?100µg/cm²). Pure 100% nitrocellulose exhibits the highest sensitivity with very low backgrounds in all transfers, especially in protein blotting. Unlike PVDF, nitrocellulose wets out naturally, does not require methanol, and will not turn hydrophobic during the transfer process. Nitrocellulose is easily blocked and does not need the many blocking steps required with PVDF. Excellent results will be obtained with all detection systems; antibody/antigen, radiolabelled, biotinylated, and chemiluminescent, allowing for a great amount of flexibility in designing a procedure. All membranes are Triton-free and cytotoxin-free (surfactant-free)

Immobilize by baking or UV crosslinking.