Easy-to-use, Colorimetric System
Hydrogen peroxide is a part of the oxygen reduction pathway, produced by the two-electron reduction of molecular oxygen, or by the one electron reduction of superoxide anion radical. Hydrogen peroxide is a potent oxidant, and the levels of hydrogen peroxide must be accurately determined in order to fully characterize the oxidative state of the system under study.National Diagnostics Hydrogen Peroxide Assay Kit is a rapid, sensitive and quantitative method for the determination of Hydrogen Peroxide in chemical or biological systems. The assay is based upon formation of a complex between Xylenol Orange and ferric iron, which is produced by the peroxide dependent oxidation of ferrous iron. This reaction is quantified colorimetrically, detecting as little as 15ng/ml of peroxide.
National Diagnostics Hydrogen Peroxide Assay is the ideal complement to our well established Diogenes Superoxide detection system. Using these kits in tandem, researchers can now quantitatively determine the first two steps in the oxygen reduction pathway.
Protocol
Assay Reagent Preparation
Prepare 1.8ml of reagent per sample to run tests in duplicate.
1. To prepare 20ml: Combine 19.8ml of Component A with 0.2ml
Component B.
2. The mixture is stable at room temperature for one working day.
Assay Procedure
1. Mix 0.9ml of Assay Reagent with up to 0.1ml of sample.
2. Incubate at room temperature for at least 30 minutes to allow
for complete color development.
3. Read absorbance at 560nm.