• Predicted molecular weight: 17.2 kDa
• No endotoxins, TAGS, and carrier-free
• Naturally folded and post-translationally modified

IBI recombinant proteins are expressed inPichia pastoris(yeast) system which provides many advantages overE. colisystems. Yeast systems promote proper protein folding and post-translational modifications for greater bio-activity. The recombinant proteins are endotoxin free and are purified without HIS-TAGS or any other TAG. IBI’s yeast expression system has proven to produce more active proteins that more closely resemble their host form.

Tumor necrosis factor alpha (TNFSF2) is a member of the TNF Superfamily. It is produced chiefly by activated macrophages, but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac myocytes, adipose tissue, fibroblasts, and neuronal tissue. The primary role of TNF alpha is in the regulation of immune cells. TNF alpha, being an endogenous pyrogen, is able to induce fever, to induce apoptotic cell death, to induce sepsis (through IL-1 & IL-6 production), to induce cachexia, induce inflammation, and to inhibit tumorigenesis and viral replication.

The recombinant human protein TNF alpha has a predicted molecular weight of 17.2 kDa.

All IBI recombinant proteins are lyophilized and shipped at room temperature without carrier protein. Proteins can be reconstituted in sterile PBS with at least 0.1% of carrier protein such as BSA or cell assay media.

The TNF alpha human protein can be used in cell culture, as a TNF alpha ELISA Standard, and as a Western Blot Control.