• Predicted molecular weight: 14.2 kDa
• No endotoxins, TAGS, and carrier-free
• Naturally folded and post-translationally modified

IBI recombinant proteins are expressed inPichia pastoris(yeast) system which provides many advantages overE. colisystems. Yeast systems promote proper protein folding and post-translational modifications for greater bio-activity. The recombinant proteins are endotoxin free and are purified without HIS-TAGS or any other TAG. IBI’s yeast expression system has proven to produce more active proteins that more closely resemble their host form.

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes and monocytes. GM-CSF is secreted in response to inflammatory stimuli such as LPS, IL-1, and TNF-α by a variety of different cells, including endothelium, fibroblasts, muscle cells, and macrophages, and by activated T cells. GM-CSF is glycosylated in its mature form.

The recombinant mouse protein GM-CSF has a predicted molecular weight of 14.2 kDa.

The biological activity of recombinant mouse GM-CSF was measured in a cell proliferation assay using the mouse FDC-P1 cell line. The ED50 for this effect is typically 0.0015 - 0.0023ng/mL.

All IBI recombinant proteins are lyophilized and shipped at room temperature without carrier protein. Proteins can be reconstituted in sterile PBS with at least 0.1% of carrier protein such as BSA or cell assay media.

The GM-CSF mouse protein can be used in cell culture, as a GM-CSF ELISA Standard, and as a Western Blot Control.

Country of Origin: USA