Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent-to-sample ratio used, giving the user flexibility to perform left, right or double-sided size selection. This product is designed for both manual and fully automated purification of DNA and RNA samples, as well as for the purification of PCR products. The system combines Omega Bio-tek’s proprietary chemistries with reversible nucleic acid-binding properties of magnetic beads to selectively bind fragments and eliminate excess nucleotides, primers, and small, non-targeted products such as primer-dimers. Purified DNA and RNA is suitable for a variety of downstream applications such as NGS library preparation, microarrays, automated fluorescent sequencing and restriction enzyme digestion.
- No protocol change against major competitor
- NGS: Double or single-sided size selection
- DNA cleanup: PCR cleanup
- RNA cleanup: cDNA or RNA purification
- Manual or adaptable to most open-ended liquid handlers
- Significant cost savings
- 96- or 384-well formats
Protocols are available for the following automated platforms:
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|
| Downstream application | Cloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 15 µL or above |
| Starting material | DNA or RNA: PCR products, gDNA, cDNA |
| Starting amount | Scalable |
| DNA recovered | >90% recovery for DNA >100 bp |
| Processing mode | Automated; manual |
| Throughput | 96-384 samples per run |
| DNA binding technology | Magnetic beads |
| Storage | 2°C - 8°C |
| Special note | Size selection by varying beads ratio |
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1.) Materials and Equipment Needed to be supplied by End-User. For 96-well plate Protocol:
• 96-well PCR plate containing PCR samples (up to 50 μL/well)
• Magnetic separation device (Recommend AlpAqua Cat# 001322). For elution volumes <30 µL AlpAqua Magnum FLX (Cat#A000400) is recommended.
• 96-well microplate or PCR Plate for elution
• Vortexer
• Multichannel pipettor
• Multichannel disposable reservoirs
• Sealing Film (#ST-3094)
• 70% ethanol
• Elution Buffer (Cat#PDR048 or 10 mM Tris pH 8.0),TE Buffer, or nuclease-free water
2.) Materials and Equipment Needed to be supplied by End-User. For 384-well plate Protocol:
• 384-well PCR plate containing PCR samples (up to 100 μL/well)
• Magnetic separation device for 384-well PCR plates
• Skirted 384-well PCR plate
• Vortexer
• Multichannel pipettor
• Multichannel disposable reservoirs
• Sealing film (#ST-3094)
• 70% ethanol
• Elution Buffer (Cat#PDR048 or 10 mM Tris pH 8.0),TE Buffer, or nuclease-free water