The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit offers a versatile solution for the isolation of high-quality DNA from a wide variety of samples, including fresh or frozen cultured cells and tissues, buccal swabs, mouse tail snips, dried blood spots, up to 250 µL whole blood, and up to 500 µL saliva.
Our cutting-edge Mag-Bind® technology reduces overall processing time by providing a quick magnetic response. This Kit combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with our proprietary chemistries to provide a rapid and reliable method for DNA isolation from a variety of samples. Purified DNA is high-quality and suitable for use in myriad downstream applications, including PCR, NGS, and enzymatic reactions.
Ready-to-implement protocols are available for the following platforms:
- Accuris IsoPURE™ 96
- Hamilton Microlab® STAR
- Hamilton Microlab® NIMBUS
- Tecan Fluent®
- Tecan Freedom EVO®
- Thermo Fisher Scientific KingFisher® Apex
- Thermo Fisher Scientific KingFisher® Flex
| FEATURES | SPECIFICATIONS |
|---|
| Starting Amount | 100-250 μL blood samples, saliva, swabs, mouse tails, dried blood spots or 5 x 106 cultured cells. |
| Elution Volume | 50-200 µL |
| Technology | Magnetic Beads |
| Processing Mode | Automated, Manual |
| Throughput | 8 – 384 |
| Note | Downstream applications: NGS, qPCR, microarray |
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Materials and Reagents that need to be Supplied by the End-User:
• 100% ethanol
• 100% isopropanol
• Nuclease-free water
• Magnetic separation device (Recommend AlpAqua Magnum™ EX, Part#A000380)
• Vortexer
• Heat block, incubator, or water bath capable of 70°C
• 96-well Microplate (500 μL) (Cat# EZ9604) or desired elution plate
• 2 mL 96-well deep-well plates (Recommend Nunc, Part#278752) or
desired plate compatible with the magnetic separation device
• Multichannel pipettes and reagent reservoirs
• Sealing film (Cat# AC1200)
• Optional: RNase A (10 mg/mL)
• Optional: PBS
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