Isolate DNA from tissues, buccal swabs, cultured cells, whole blood, body fluids, paraffin-embedded tissues and mouse tail snips using mini spin columns

Omega Biotek E.Z.N.A.® Tissue DNA Kit offers a simple, rapid, and cost-effective method for the isolation of DNA from a wide variety of sample sources include fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in fewer than 30 minutes. Up to 30 mg of tissue at a time can be easily processed using the simple E.Z.N.A.® Tissue DNA protocol. With the spin-column based kit, multiple samples can be processed in parallel. There is no need for phenol/chloroform extractions or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® Tissue DNA Kit is ready for most downstream applications such as PCR, Southern blots, and restriction enzyme digestion.

• Rapid – DNA isolation in under 20 minutes (after lysis)
• Reliable – Optimized buffers guarantee pure DNA every time
• Safe – No organic extractions
• High-quality – Purified DNA suitable for most applications

Performance Comparison of E.Z.N.A.® Tissue DNA Kit Against Leading Competitors

Figure 1.  Purified genomic DNA from 10 mg rat kidney tissue was isolated using kits from Company T, Company A, Company P, Company Q, and Omega Bio-tek’s E.Z.N.A.® Tissue DNA Kit following manufacturer’s recommended protocols. 3% of eluted DNA was analyzed on a 0.8% agarose gel. M:Lambda-Hind III.

Real-time PCR of Genomic DNA Isolation with E.Z.N.A.® Tissue DNA Kit

Figure 2.  Genomic DNA was isolated from 10 mg of rat kidney with Omega Bio-tek’s E.Z.N.A.® Tissue DNA Kit. Serial dilutions of recovered genomic DNA were used as templates for real-time PCR amplification of a 100 bp fragment of the GAPDH gene with SYBR® Green labeling. Each reaction was performed in triplicate. The fluorescence versus cycle number is plotted and the 5 curves correspond to the input DNA template amounts of 10, 2, 0.4, 0.08, and 0.0016 ng.

Genomic DNA Yield From Mouse Liver

Table 1.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples and eluted in 100 µL volume. DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop ™ 2000c system.

Intact, high-molecular weight DNA as analyzed on an Agarose Gel

Figure 3.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples and eluted in 100 µL volume. 5 µL eluate was analyzed on a 1% Agarose gel.

Real-time PCR analysis of extracted DNA

Figure 4.  Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples. 2 µL of Eluted DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

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