Omega Biotek E.Z.N.A.® FFPE Genomic DNA Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method or an efficient heat treatment. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. After adjusting the binding conditions with ethanol, the lysate is applied to the MicroElute DNA column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. High quality purified DNA is then eluted in elution buffer or water and is ready for applications such as PCR and next-generation sequencing.

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Quality and yield of DNA with agarose gel analysis comparing Company A’s FFPE Kit and Omega Bio-tek’s E.Z.N.A.® FFPE DNA Kit. Analysis was completed on a 0.5% agarose gel. Lanes 1-3 represent Company A and lanes 4-6 represent Omega Bio-tek’s E.Z.N.A.® FFPE DNA Kit. M: 1 kb marker. FFPE DNA tissue starting material was ~2 mg post-deparaffinization. An overnight digestion was completed at 55°C. Samples were eluted in 60 µL elution buffer. O.D. ratio and yield calculated using a Thermo Fisher NanoDrop® 2000c.