• FREE Script computer programs available for all popular Workstation Platforms
(Automation Liquid Handling Robotic Systems). Request from us, if necessary.
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Omega Biotek Mag-Bind® Total RNA Kit provides a novel technology for total RNA isolation. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of cells and tissues. The system combines the efficient nucleic acid-binding properties of Mag-Bind® Particles with the E.Z.N.A.® RNA isolation system to provide superior quality RNA. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, which improves removal of contaminants and increases nucleic acid purity. Mag-Bind® Total RNA procedure can be fully automated with most robotic workstations.
RNA Integrity. Total RNA was extracted from 3×106 cells using the Mag-Bind® Total RNA Kit and analyzed on Agilent’s TapeStation® 2200.
RNA Yield Comparison. RNA was isolated using the Mag-Bind® Total RNA Kit and a comparable column-based kit from Company Q. RNA was quantified using Thermo Scientific’s NanoDrop® 2000c
Mag Bind Total RNA 96 versus Company Q silica spin column based method. RNA was isolated according to manufacturer’s protocol. RNA quality assessed via Agilent Tapestation 2200 and RNA quantity determined via Thermo Nanodrop 2000c. Omega Bio-tek Mag-Bind Total RNA 96 had higher yields when comparing the amount of tissue input per extraction.
NOTE: Special Quantity and/or Standing Order pricing available. Inquire.
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Materials and Equipment needed to be Supplied by End-User:
• Centrifuge capable of 4,000 x g with swing-bucket rotor for 96-well plates
• Adapter for 96-well deep-well plates |
• Magnetic separation device
• Equipment for disrupting tissue (Geno/Grinder 2000/2010) or mortar and pestle
• Vortexer
• 100% ethanol
• 100% isopropanol • Multi-channel pipettor and nuclease-free pipette tips
• Sealing film (Recommend Cat# AC1200-01)
• Multi-channel reservoirs
• 96-well microplates with a minimum capacity of 1 mL (Recommend Cat# DW-110-5)