DNA extraction from up to 10 mL whole blood.

Omega Biotek Mag-Bind® Blood DNA HV kit is designed for rapid and reliable isolation of high-quality genomic DNA from up to 10 mL whole blood and saliva. The protocol has been successfully automated on the Hamilton Microlab® STAR™ and 24 samples can be processed in less than 2.5 hours. The system combines the reversible nucleic acid-binding properties of Mag-Bind® paramagnetic particles with the time-proved efficiency of Omega Bio-tek’s DNA isolation system to provide a fast and convenient method to isolate DNA from a variety of samples. The Mag-Bind® particles provide fast response time when applied to a magnetic field allowing for quick separation from high volumes of liquid.

DNA Yield & Quality. DNA was extracted from 10 mL of whole blood samples and was eluted in 2 mL volume. The DNA yield was determined using Thermo Scientific’s NanoDrop™ 2000c and Promega’s QuantiFluor® dsDNA system. The absorbance at different wavelengths was determined by NanoDrop™ spectrophotometer.

TapeStation® Analysis of Purified DNA.TapeStation® analysis of genomic DNA purified from 10 mL of whole blood sing Omega Bio-tek’s Mag-Bind® Blood DNA HV Kit automated on Hamilton’s Microlab® STAR™.

Real-time PCR on Serial Dilutions of Purified DNA. Average Ct values obtained using human-specific primers on 1X, 10X and 100X dilutions of purified DNA used as the template. Sample IDs 1-6 are represented here.