Omega Biotek Mag-Bind® Plant DNA DS 96 Kit, for Plant DNA Extractions, allows rapid and reliable isolation of high-quality genomic DNA from plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content. The lysis and binding buffers are specifically designed to minimize co-purification of polysaccharides and polyphenols. Up 96 samples of 50 mg wet tissue (or 15 mg dry tissue) can be processed in parallel in less than one hour. The system combines CTAB-based lysis, which eliminates the need for organic solvents, with the convenience of Mag-Bind® Particles to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. Purified DNA is suitable for PCR, restriction digestion, next generation sequencing, and hybridization applications. There are no organic extractions thereby reducing consumables and decreasing hands-on time to allow multiple samples to be processed in parallel.

• Straightforward, rapid, and reliable procedure
• Optional rebind step provides optimal purity for complex samples
• Adaptable in most robotic liquid handling platform

Approximately 50 mg leaf sample extracted per sample according to manufacturer’s recommended protocols. DNA concentration determined via fluorescence-based nucleic acid quantification. DNA quantification confirmed via SYBR® qPCR (data not shown). Amount of DNA per mg of leaf sample shown above.