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Omega Bio-tek’s Mag-Bind® RxnPure Plus Kit allows rapid and reliable isolation DNA from PCR* and other enzymatic reactions with high yield. The system combines Omega Bio-tek’s proprietary DNA extraction chemistries with the reversible nucleic acid-binding properties of magnetic beads that selectively bind PCR amplicons 100 bp and larger and eliminate excess nucleotides, primers, and nonspecific products such as primer dimers.
This kit is designed for both manual and fully automated protocols and may not require reprogramming of liquid handling instruments depending on your current method.
The Mag Bind RxnPure Plus is compatible with multiple liquid handlers and magnetic processors such as Hamilton STAR/STARlet and Beckman Coulter Biomek FX/NX.
- Removes excess primers, primer-dimers, dNTPs and salts
- Does not require centrifugation/filtration steps
- Can be adapted and scaled to most standard liquid handling robots
- Available in 96 or 384 well format
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Materials and Equipment Needed to be Supplied by End-User for 96-well Protocol:
• 96-well PCR plate containing PCR samples (up to 50 μL/well)
• Magnetic Separation Device (Recommended Cat# AlpAqua A001322)
• Vortexer
• Multichannel pipettor
• Multichannel Disposable Reservoirs
• Sealing film (ST-3094)
• 96-well microplate for elution
• 70% ethanol
• Elution Buffer (Cat#PDR048 or 10 mM Tris pH 8.0) or TE Buffer (10 mM Tris pH 8.5)
• Optional: Oven capable of 37°C
Materials and Equipment Needed to be Supplied by End-User for 384-well Protocol:
• 384-well PCR plate containing PCR samples (up to 100 μL/well)
• Magnetic separation device for 384-well PCR plates
• Vortexer
• Multichannel pipettor
• Skirted 384-well PCR plate
• Multichannel Disposable Reservoirs
• Sealing film (#ST-3094)
• 70% ethanol
• Elution Buffer (Cat#PDR048 or 10 mM Tris pH 8.0) or TE Buffer (10 mM Tris pH 8.5)
• Optional: Oven capable of 37°C