The Mag-Bind® Universal Pathogen DNA 96 Kit allows rapid and reliable isolation of high-quality pathogen and host genomic DNA from tissue samples. Up to a 30 mg tissue sample is recommended and can be processed in less than 60 minutes. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.
The system combines the Mag-Bind® technology with RBB Buffer to eliminate PCR inhibiting compounds within the samples. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. There are no organic extractions, thus reducing plastic waste and hands-on time and multiple samples can be processed in parallel.
Cryptosporidium oocysts were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific for the target organism. Average of triplicate data shown.
Group B strep cultured samples were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.
E. coli cells were cultured overnight in LB broth and added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.
HBV viruses were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.
Influenza A/B virus was added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.