You have no items in your shopping cart.

Omega Biotek E.Z.N.A. Bacterial DNA Mini Kit allows the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of gram positive and negative bacterial species. This kit uses optimized lysis condition and up to 1 x 10^9 bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

  • Reliable – Reproducible DNA purification from variety of sample sources
  • High yield – Glass beads and enzymatic digestion for cell lysis

 
Genomic DNA purified using the E.Z.N.A.® Bacterial DNA Kit. Purified genomic DNA from various bacteria were isolated with the E.Z.N.A.® Bacterial DNA Kit. DNA was extracted in duplicate from each of the following samples: sample 1  from S. aureus, sample 2 from B. subtilis and sample 3 from P. aeruginosa. Genomic DNA (5% of total purified DNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting Amount1 x 109 cells
Starting MaterialBacterial cells
Yield30 µg
Elution Volume50-100 μL
TechnologyHiBind® DNA Mini Column
Processing ModeManual
Throughput 


.  .  .  SAVE  .  .  . by ordering 4 kits

Omega Biotek E.Z.N.A. Bacteria, and Yeast DNA Mini Kit, 200 preps

Yeast and Bacteria - gDNA . . . - SAVE - by ordering 4 kits
E.Z.N.A. Bacteria DNA Mini Kit, 200 prep
(Description below. Register for discount.)
SKU: D3350-02
Old price: $566.00
$474.20
PRICE BREAKS - The more you buy, the more you save
Quantity4+
Price$439.00
Products specifications
Attribute nameAttribute value
Binding FormatMini Spin Columns
Mechanical Lysis (Glass Beads)Yes
Enzymatic DigestionYes
Gram Positive & Negative BacteriaYes
PCR Inhibitor RemovalNo
AutomatableNo
Downstream ApplicationsPCR, Southern Blot, SNP