The E.Z.N.A.® Bacterial RNA Kit is designed for isolation of high quality total RNA from variety of bacterial strains. Up to 1 x 10^9 log- phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridization techniques.
Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from 2 mL bacterial samples as follows: sample 1 from Pseudomonas arginosa, sample 2 from Enterococcus faecalis, and sample 3 from Staphylococcus aureus. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA.