Omega Bio-Tek

E.Z.N.A. Blood RNA MIDI Kit

Efficient Isolation of Total RNA from Whole Blood

E.Z.N.A.® Blood RNA MIDI Kit is designed for isolation of total intracellular RNA from up to 10 ml of fresh, whole blood treated with any common anticoagulant such as heparin, EDTA, or acid-citrate-dextrose. 10 ml of blood typically yields 0.7- 1mg of total RNA. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient, and reliable. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl, are eliminated. The kit is also suitable for isolation of total RNA from cultures cells, tissues, bacteria, and from RNA viruses.

RNA purified using the E.Z.N.A.® Blood RNA method is ready for applications such as RT-PCR*
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This kit eliminates the need for hazardous phenol/chloroform extraction, and time consuming steps such as CsCl gradient ultracentrifugation and precipitation with isopropanol or LiCl.

These Blood RNA kits use the reversible properites of HighBind silica matrix combined with the speed of mini-column Spin technology. Red blood cells are selectively lysed and white cells collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, total RNA is purified in the HighBind matrix spin column. A specially formulated high salt buffer system allows RNA molecules greater than 200 bases to bind to the matrix. Cellular debris and other contaminants (such as hemoglobinn) are effectively washed away and high quality RNA is finally eluted in DEPC treated sterile water. Purified RNA is suitable for most applications including RT-PCR. Kit protocols include an optional on-column DNase digestion procedure.

RNA Isolation and Purification

Manipulation of RNA is inherently more challenging than that of DNA since RNase is not only ubiquitous to most laboratories, it is also extremely unstable. Omega RNA EZ-Kits and EZ-96 plate Kits allow for reliable isolation of RNA from a variety of sources with its HighBind spin column method. These systems involve the simultaneous lysis of cells from a variety of sources of endogenous RNases. This is accomplished by using proprietary denaturing buffers that enhance the reversible binding of RNA to the HighBind silica matrix. While cells can be lysed directly, tissues are disrupted or homogenized using common laboratory supplies. Binding conditions are further adjusted and lysates are applied to the spin columns that selectively bind RNA molecules greater than 200 nt in length, thus favoring mRNA isolation. Successive washes with specially formulated buffers remove inhibitors and contaminants, allowing subsequent elution of pure RNA with water. As with other Omega products, phenol and chloroform extraction are eliminated. Kits are tailor made for specific cells and tissue sources to ensure consistent and reliable RNA preps, every time.