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Mini sized Blood RNA Isolation Kit (Homogenizer Columns included)

- Compare with QIAGEN’s QIAamp® #52304 -

Efficient Isolation of Intracellular RNA from Whole Blood

The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 mL of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNase’s, the lysate is homogenized with a homogenizer spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as hemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC-treated water.


 
Total RNA purified using the E.Z.N.A.® Blood RNA Kit.Purified total RNA from various blood was isolated with the E.Z.N.A.® Blood RNA Kit. RNA was extracted from blood samples as follows: sample 1 from 200 µL human blood, sample 2 from 400 µL human blood, sample 3 from 1 mL human blood, sample 4 from 100 µL mouse blood,  and sample 5 from 200 µL mouse blood. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.
 

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Downstream ApplicationPCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume50-100 μL
Starting MaterialWhole blood; animal tissue; others with modified protocols
Starting AmountUp to 1 mL blood; up to 30 mg tissue
RNA YieldUp to 100 µg
Processing Modemanual, centrifugation
Throughput1 - 24
RNA Binding TechnologySilica Mini spin column
Binding Capacity100 µg

Omega Biotek Blood RNA Mini Kit, 50 preps

Blood and Viral - RNA Kits
E.Z.N.A. Blood RNA Mini Kit, 50 preps
(Description below. Register for discount.)
SKU: R6814-01
Old price: $294.20
$255.90
Products specifications
Attribute nameAttribute value
Starting Sample Volume10-1000 μl
Binding FormatMini Spin Columns
Homogenizer Columns IncludedYes
Red Blood Cell Lysis Step requiredYes
Includes DNase TreatmentNo
AutomatableNo
Organic Extractions RequiredNo