The E.Z.N.A.® Cycle-Pure Kit is designed for the rapid purification of single or double-stranded DNA from PCR or other enzymatic reactions. The purification procedure completely removes primers, nucleotides enzymes, salts, and other impurities from DNA sample. The DNA sample is simply mixed with buffer and spun through the HiBind DNA column. The DNA bound to the HiBind Matrix is washed and the clean concentrated DNA is eluted with deionized water or elution buffer. This convenient spin-column format eliminates the need for expensive resins or toxic organic compounds such as phenol and chloroform, thereby making it possible to process multiple samples in parallel. Purified DNA can be used in T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions.
The E.Z.N.A.® Cycle Pure Kit is is available with 2 different types of columns: V-spin columns have an attached cap (D6492), while Q-spin columns are capless (D6493). The columns are otherwise identical in use and application.
- Rapid – Purification of PCR products in 10 minutes
- Convenient – No Phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for downstream applications
Purified DNA from a 500 bp and 5 kb fragment was purified with Company T, Company A, Company P, Company Q, and Omega Bio-tek’s Cycle Pure Kit following each manufacturer’s recommended protocols. 10% of eluted DNA was analyzed on a 0.8% agarose gel.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|
| Downstream application | T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions |
| Elution volume | 30-50 µL |
| Starting material | ssDNA, dsDNA, PCR products |
| Starting amount | 5-50 µL |
| DNA recovered | >90% recovery, 100 bp to 10 kb |
| Processing mode | Manual (centrifugation or vacuum) |
| Throughput | 1-24 |
| DNA binding technology | Silica mini spin column |
| Processing time | <10 minutes |
| Special Notes | For clean-up of DNA fragments < 200bp, please follow the protocol modification outlined in the product manual |
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Compare with Qiagen® 28104 and 28106