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The E.Z.N.A.® Cycle-Pure Kit is designed for the rapid purification of single or double-stranded DNA from PCR or other enzymatic reactions. The purification procedure completely removes primers, nucleotides enzymes, salts, and other impurities from DNA sample. The DNA sample is simply mixed with buffer and spun through the HiBind DNA column. The DNA bound to the HiBind Matrix is washed and the clean concentrated DNA is eluted with deionized water or elution buffer. This convenient spin-column format eliminates the need for expensive resins or toxic organic compounds such as phenol and chloroform, thereby making it possible to process multiple samples in parallel. Purified DNA can be used in T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions.

The E.Z.N.A.® Cycle Pure Kit is is available with 2 different types of columns: V-spin columns have an attached cap (D6492), while Q-spin columns are capless (D6493). The columns are otherwise identical in use and application.

  • Rapid – Purification of PCR products in 10 minutes
  • Convenient – No Phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for downstream applications

Purified DNA from a 500 bp and 5 kb fragment was purified with Company T, Company A, Company P, Company Q, and Omega Bio-tek’s Cycle Pure Kit following each manufacturer’s recommended protocols. 10% of eluted DNA was analyzed on a 0.8% agarose gel.
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Compare with Qiagen® 28104 and 28106

Omega Biotek PCR Cycle Pure Clean-Up Kit (with V-spin Columns), 5 preps

PCR CleanUp
PCR Cycle Pure Kit, V-spins, 5 preps
(Description below. Register for discount.)
SKU: D6492-00
$16.80
Products specifications
Attribute nameAttribute value
Silica Column/Plate BasedYes
Magnetic Bead BasedNo
Available in: Q spin (capless) and V spin (capped)Yes
96 Well Format ProcessingNo
384 Well Format ProcessingNo
Recovery Size Range100 bp- 20 kb
Elution Volume30-50 μl
Binding Capacity25 μg
AutomatableNo