Omega Biotek E-Z 384 Plant DNA HT Kit
Omega Biotek's E-Z 384 Plant DNA HT Kit enables rapid and high–throughput isolation of genomic DNA from a wide variety of plant tissue samples. (NOTE - Processing samples does NOT require the use of an expensive Liquid Handler or Magnetic Processor.) Up to 768 fresh, frozen, or dried plant tissue samples rich in polyphenols, polysaccharides or having a lower DNA content can be processed in approximately 1.5 hours, when processing two plates in parallel. The system combines the reversible nucleic acid-binding properties of our HiBind® silica matrix with the speed and versatility of a 384-well format to maximize sample processing throughput. The purification procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with a unique binding system to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue samples. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. The eluted DNA is inhibitor-free and ready for downstream applications such as genotyping, plant breeding, population level analyses, Next Generation Sequencing, PCR, and more.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|
| Starting Material | Fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a low DNA content |
| Starting Amount | 30 mg fresh/10 mg dry plant sample per well |
| Elution Volume | 65 µL |
| Processing Mode | Manual (Centrifugation or Vacuum) |
| Throughput | 1 - 768 (process two 384-plates in parallel) |
| Nucleic Acid Binding Technology | 384-well silica filter plate |
| Processing time | 1.5 h |
| Downstream Application(s) | Genotyping, plant breeding, population level analyses, next generation sequencing, PCR, and more. |
Travis Butts, Vice President of Omega Bio-tek says, “The E-Z 384 Plant DNA HT Kit was designed to address laboratories in the agriculture industry that need to process large volumes of plant samples rapidly and efficiently, but do not have access to liquid handlers or magnetic processors. Large volume sample processing has been a significant bottleneck in performing population-level genomic studies. With this high-throughput kit, we aim to ease that and enable systems-based approaches for plant research.”