Isolates up to 1ml endo-free grade plasmid DNA from 50-200 mL cultures in 45 minutes.

The E.Z.N.A.® Endo-Free FastFilter Plasmid Maxi Kits provide an efficient method for the large-scale isolation of endotoxin-free plasmid DNA. Obtain clear cell lysate using a syringe method versus centrifugation. 

Following lysis, endotoxins are removed from the cleared lysate with the addition of ETR Solution. Plasmid DNA is then further purified by adsorbing to the HiBind silica membrane. An easy wash step is followed by the elution of the bound plasmid DNA in elution buffer. Up to 200 µg of high-quality plasmid DNA can be purified from 30-50 mL of overnight culture (D6915), and up to 1 mg from 100-200 mL of overnight culture (D6926). Purified DNA samples are ready to be used in downstream applications such as, automated fluorescent DNA sequencing, restriction enzyme digestion, transfection of mammalian cells, cloning, and PCR amplification.

• Speed – Purification of plasmid DNA in < 45min
• Safety – No Phenol/chloroform extractions
• Quality - Endotoxins efficiently reduced (<0.1 EU/µg)

Applications: Plasmid DNA Isolation Kits are used for screening, restriction endonuclease digestions, manual or automated fluorescent DNA sequencing, transfection, ligations, and other manipulations.