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Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid Mini Kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0.1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

  • Speed – 30 minutes or less
  • Safety – No Phenol/chloroform extractions
  • Versatility – Spin and vacuum formats available
  • Quality - Endotoxins efficiently reduced (<0.1 EU/µg)

Omega Biotek Endotoxin-Free Plasmid DNA Mini-Kit II, 200 preps

Endo-Free Plasmid DNA Mini II, 200 prep
(Description below. Register, get discount.)
SKU: D6950-02
Old price: $456.10
$382.50
Products specifications
Attribute nameAttribute value
Sample Size1-15mL
Binding Capacity70 μg
Processing Time40 min
Plasmid TypeHigh & Low Copy Plasmids
Starting Amounts5-10 mL
Restriction Enzyme, Sequencing, PCR, TransformationYes
Sensitive Cell Line & Primary Cell TransfectionYes
Transfection, In vitro transcriptionYes
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