E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase Free HiBind® DNA Midi Columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 mL of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids, one can increase the starting culture volume up to 100 mL. High-quality DNA is ready for immediate use in routine molecular biology applications such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.
- Rapid – Purification of plasmid DNA in < 60 min
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for most downstream applications
Yield: 100-250 µg for high copy-number; 10-50 µg for low copy-number
Starting material: 15-50 mL LB culture with OD600 between 2 and 3; or equivalent
Up to 100 mL of culture may be processed when working with low copy number plasmid. |