Omega Biotek  E.Z.N.A.® Yeast DNA Kit is comprised of a quick and efficient lysis procedure to isolate of high-quality genomic, YAC, or plasmid DNA from yeast. There are no organic extractions, thus reducing plastic waste and hands-on time to allow for reliable isolation of total cellular DNA from a wide variety of yeast species. Purified DNA is suitable for PCR, restriction enzyme digestion, and hybridization techniques to be processed in parallel.

Yield approximately 15-30µg of DNA with an A260/A280 ratio of 1.7-1.9 in less than 20 minutes.

• Reliable – Reproducible DNA purification from variety of sample sources
• High yield – Glass beads and enzymatic digestion for cell lysis

Genomic DNA purified using the E.Z.N.A.® Yeast DNA Kit.Purified genomic DNA was isolated with the E.Z.N.A.® Yeast DNA Kit. Various yeast was cultured in 3 mL PDA culture medium for 20 hours for each of the following samples: sample 1 from S. cerevisae, sample 2 from P. pastoris, sample 3 from Candida rugosa, sample 4 from R. glutinis, and sample 5 from Nematspora gossypii. Genomic DNA (10% of total purified DNA) was analuzed on a 0.8% agarose gel to demonstrate yield and quality of the DNA.