Using superior Magnetic Bead Technology, the MagBind SeqDTR kit removes unincorporated dye terminators from sequencing reactions.
Compare with BigDye® Xterminator™*.Omega Bio-tek’s Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek’s proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter’s Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups.
- No protocol change against major competitor
- Read lengths averaging over 800 bps (Min Phred 20)
- Manual or adjustable to automated liquid handlers
- Significant cost savings compared to sephadex-based clean ups
- 96- or 384-well formats
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Comparison of magnetic bead-based big dye clean up chemistries. Purified 1.8 kb PCR fragments were sequenced from each company using the manufacturer’s recommended protocols. The median of 16 samples per company are used in the representations above. A 5 µL sequencing reaction was performed using a 1/32 dilution of Applied Biosystems BigDye Terminator v3.1 chemistry. DNA was analyzed on an Applied Biosystems 3730XL.
DNA samples treated with Mag-Bind® SeqDTR had superior signal strength and continuous read length than using leading competitors.
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Cost Comparison When Using Mag-Bind SeqDTR for Sequencing Clean-up (2021)
CLICK: View Handbook for complete protocol information.
* BigDye is registered trademark owned by Applied Biosystems, Life Technology, and ThermoFisher.
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Note, this item requires a 2 day air delivery.
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Materials and Equipment Needed to be Supplied by End-User for 96-well Protocol:
• 85% ethanol (do not use denatured ethanol)
• Magnetic separation device compatible with 96-well PCR plates
• Multichannel pipet
• Reservoirs
• 96-well plate capable of being used in sequencers
• Elution Buffer (Cat# PDR048 or 10 mM Tris pH 8.5, TE Buffer, 0.1 mM EDTA, or diH₂O)
Materials and Equipment Needed to be Supplied by End-User for 384-well Protocol:
• 85% ethanol (do not use denatured ethanol)
• Magnetic separation device compatible with 384-well microplates
• Multichannel pipet
• Reservoirs
• 384-well plate capable of being used in sequencers
• Elution Buffer (Cat# PDR048 or 10 mM Tris pH 8.5, TE Buffer, 0.1 mM EDTA, or diH₂O)
Use a Magnetic Separation Device with this kit.
#MSD01B (for 96-well PCR plate) or #MSD02 (for 2ml tubes)
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