For purification of microRNA (miRNA) and Total RNA from wide range of samples including animal tissue, Cultured cells, plant, bacteria (for <200 nucleotides).
The E.Z.N.A.® Micro RNA Kit (miRNA) combines the reversible binding properties of HiBind® matrix, a superior silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials.
Micro and large RNA are separated to allow for quantification into downstream applications.
The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is application to a HiBind® RNA column where large RNA binds. The flow through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
miRNA purified using the E.Z.N.A.® miRNA Isolation Kit. Purified large RNAs and miRNA from tissue samples was isolated with the E.Z.N.A.® miRNA Isolation Kit. RNA was extracted from samples as follows: sample 1 from fish liver, sample 2 from frog liver. Large RNA (5% of total purified RNA) was analyzed on a 1% agarose gel. miRNA (5% of total purified RNA) was analyzed on a 3% agarose gel to demonstrate yield and quality of the RNA.
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|
| Downstream Application | RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation |
| Elution Volume | 15-30 μL for miRNA; 40-70 μL for >200 nt |
| Starting Material | Cultured eukaryotic cells or bacteria, or from animal,
plant, or fungal tissues |
| Starting Amount | Up to 100 mg |
| RNA Yield | Up to 50 µg |
| Processing Mode | Spin column |
| Throughput | 1 - 24 |
| RNA Binding Technology | Silica technology |
| Binding Capacity | 100 µg for each |
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Compare with Qiagen® miRNeasy Mini Kit, #217004.
The E.Z.N.A. miRNA Isolation kit uses a rapid procedure to isolate small RNAs, such as micro RNA(miRNA), small interfering RNA(siRNA), and small nuclear RNA (smRNA), from wide range of tissues and cells. The fast and efficient silica membrane (aka glass fiber filter) based method isolates total RNA ranging in size from kilo based down to 10mers. The kit also provides reagents and a procedure to enrich the population of RNAs than are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridization, Northern analysis, and other methods.
HazMat fee applies. |