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The E.Z.N.A.® MicroElute RNA Clean Up Kit is designed to purify and concentrate RNA (from 50 µg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following the phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications requiring highly concentrated RNA samples.

  • Recovery of RNA from enzymatic reactions in 15 minutes
  • No organic extractions
  • Clean up RNA isolated by others methods (e.g. crude preps from organic extraction methods)
  • Concentrates small amounts of RNA to 10-15 µL

 
Total RNA purified using the MicroElute RNA Clean Up Kit.Total RNA was purified from 15 µL RNA original solution and eluted in 15 µL elution buffer. Total RNA (2 µL) was analyzed on a 1% agarose gel. Lanes 1-4: after purification, C: before purification.

Compare with QIAGEN®s RNeasy® MiniElute® Cleanup Kit #74204

Protocol

Suitable for downstream applications including RT-PCR, Northerns, poly A+ RNA (mRNA), nuclease protection, and in vitro translation.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Downstream applicationCloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume10-15 µL
Starting materialEnzymatic reaction
Starting amountUp to 10 µg RNA
DNA recovered>90% recovery, RNA > 200 bases
Processing modeManual, centrifugation
Throughput1
DNA binding technologySilica MicroElute spin column
Binding capacity10 μg
Processing time<15 mintues
Special noteLow elution volume, suitable for RNA desalting too



Note: Replaces #R6249-series kits

Omega Biotek MicroElute RNA Clean-Up Kit, 50 preps

DNA and RNA CleanUp
MicroElute RNA Clean-Up kit, 50 preps
(Description below. Register for discount.)
SKU: R6247-01
Old price: $175.60
$146.40
Products specifications
Attribute nameAttribute value
Silica Spin ColumnYes
Magnetic Bead BasedNo
Processing Time10 min
Binding Capacity10 μg
Elution Volume10-15 μl
Post Phenol Clean UpYes
Enzymatic Reaction Clean UpYes
Concentrating SamplesYes
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