Omega Biotek
E.Z.N.A. Mollusc RNA Mini Kit
(Homogenizer Columns included)
Isolation of Total RNA from Molluscs, Arthropods, Roundworms, Flatworms, & other Invertebrate Tissue Samples.
Omega Biotek Mollusc Total RNA Isolation Mini Kits are designed for efficient recovery of total RNA greater than 200 nt from molluscs, arthropods, roundworms, flatworms, and other invertebrate tissue rich in mucopolysaccharides. The procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), inconjunction with the selective RNA binding properties of our proprietary silica-based HighBind matrix spin columns. Salts, proteins, and other contaminants are removed to yield high quality total RNA in less than 30 minutes Yield is 10-59 μg. Purified RNA is suitable for downstream applications including reverse transcription, poly A+ mRNA selection, and hybridization techniques.
Total RNA purified using the E.Z.N.A.® Mollusc RNA Kit. Purified total RNA from various samples was isolated with the E.Z.N.A.® Mollusc RNA Kit. RNA was extracted from samples as follows: sample 1 from fire ants, sample 2 from earthworms, sample 3 from snails, sample 4 from abalone, sample 5 from slug, and sample 6 from winkles. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.
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Overview: Samples are homogenized and lysed in a high salt buffer containing CTAB, and extracted with chloroform to remove mucopolysaccharides and to denature proteins. Following a rapid alcohol precipitation step, binding conditions are adjusted and RNA is further purified using our Kit HiBind silca-based spin columns.
RNA Isolation and Purification
Manipulation of RNA is inherently more challenging than that of DNA since RNase is not only ubiquitous to most laboratories, it is also extremely unstable. Omega RNA Kits allow for reliable isolation of RNA from a variety of sources with its HiBind spin column method. These systems involve the simultaneous lysis of cells from a variety of sources of endogenous RNases. This is accomplished by using proprietary denaturing buffers that enhance the reversible binding of RNA to the HighBind silica matrix. While cells can be lysed directly, tissues are disrupted or homogenized using common laboratory supplies. Binding conditions are further adjusted and lysates are applied to the spin columns that selectively bind RNA molecules greater than 200 nt in length, thus favoring mRNA isolation. Successive washes with specially formulated buffers remove inhibitors and contaminants, allowing subsequent elution of pure RNA with water. As with other Omega products, phenol and chloroform extraction are eliminated. Kits are tailor made for specific cells and tissue sources to ensure consistent and reliable RNA preps, every time.