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Picture of 200 Prep (200ml) - Omega Biotek RNA-Solv Reagent Kit (With Phenol)
RNA-Solv Reagent
Single reagent RNA Isolation System
 (TRIzol® alternative)
Omega Biotek RNA-Solv Reagent is an one-reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine thiocyanate, is a modified version of the single-step RNA isolation method that is commonly used in many research labs. The sample is homogenized and lysed in RNA-Solv Reagent which maintains the integrity of RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase and is subsequently recovered by isopropanol.

This method is suitable for small quantities of tissue (<100 mg) and cells (5 x 106), as well as large quantities of tissue (up to 1g) and cells (1 x 108) of human, animal, plant or bacterial origin. The simplicity of the RNA-Solv Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Purified RNA can be used for Northern blot analysis, RT-PCR, Poly A+ (mRNA) selection, in vitro translation, and RNase protection assays.
 Hazardous and Dry Ice Fee applies.
 
Features and Benefits
  • Simple - One-reagent system
  • Flexible - Solution-based method, easily scaled up and down

Inquire for MSDS and/or Manual.
RNA-Solv is stable for at least 12 months when stored at 2-8C,

200 Prep (200ml) - Omega Biotek RNA-Solv Reagent Kit (With Phenol)

RNA-Solv Reagent, with Phenol
RNA-Solv Reagent, 200 prep (200ml)
(Description below. Register for discount.)

TRIzol alternative
SKU: R6830-02
$270.60
Products specifications
Attribute nameAttribute value
Starting Sample VolumeScalable
Binding FormatIsopropanol Precipitation, Isopropanol Precipitation
Isolates RNA <200 ntNo
Elution Volume>35 μl
Isolates RNA >200 ntYes
Sample TypeAll Tissue Types
Isolate mRNA directly from Cells/TissueNo
Dnase l DigestionNo
Organic Extractions RequiredYes
Can Isolate DNA,RNA & ProteinsYes
AutomatableNo
Organic ExtractionsYes
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