2 preps, Total RNA Midi Kit
Omega Biotek
E.Z.N.A. Total RNA Midi Kit
- Compare QIAGEN’s RNeasy® Midi #75142, #75144 -
Rapid Isolation of 100-500μg Total RNA from as much as 1x108 cells or 200mg Tissue
The Midi sized Total RNA Kit provides a rapid and easy method for the isolation of up to 200mg of total RNA from cultured eukaryotic cells, tissues, or bacteria. This kit allows simultaneous processing of single or multiple samples in less than 60 minutes. Normally, up to 1 x 108 eukaryotic cells or 200mg of tissue can be used in a single experiment. This Midi sized kit uses the reversible binding properties of HighBind silica matrix technology and speciialized buffers for high quality RNA isolation. The protocol includes an on-column DNase treatment procedure. Purified RNA can be used for any application, including RT-PCR, Northern blotting, nuclease protection, and in vitro translation, and other applications.
This kit replaces and eliminates hazardous phenol/chloroform extractions and time consuming steps. such as CsCl gradient ultracentrifugation and precipitation with isopropanol or LiCl.
RNA Isolation and Purification
Manipulation of RNA is inherently more challenging than that of DNA since RNase is not only ubiquitous to most laboratories, it is also extremely unstable. Omega RNA EZ-Kits and EZ-96 plate Kits allow for reliable isolation of RNA from a variety of sources with its HighBind spin column method. These systems involve the simultaneous lysis of cells from a variety of sources of endogenous RNases. This is accomplished by using proprietary denaturing buffers that enhance the reversible binding of RNA to the HighBind silica matrix. While cells can be lysed directly, tissues are disrupted or homogenized using common laboratory supplies. Binding conditions are further adjusted and lysates are applied to the spin columns that selectively bind RNA molecules greater than 200 nt in length, thus favoring mRNA isolation. Successive washes with specially formulated buffers remove inhibitors and contaminants, allowing subsequent elution of pure RNA with water. As with other Omega products, phenol and chloroform extraction are eliminated. Kits are tailor made for specific cells and tissue sources to ensure consistent and reliable RNA preps, every time.
Storage and Stability - LSPI’s Omega RNA EZ-SpinKit and EZ-96 plate Kit isolation components are guaranteed for at least 12 months from the date of purchase, when stored as recommended in supplied instruction manuals.