200 preps, V-Column spin design

OmegaBiotek

- Compare with QIAGEN’s RNeasy® #74104, #74106 -

Rapid RNA preps from Animal Cells & Tissues

The Total RNA Mini-Kit I provides a rapid and easy method for the isolation of up to 100µg of total RNA from cultured eukaryotic cells and tissues. This kit allows for the simultaneous processing of samples in less than 25 minutes. Normally, up to 1 x 10⁷ eukaryotic, up to 1 x 10⁹ bacterial cells, or 30mg of tissue can be used in a single experiment. Average yield is 10-100µg. Purified RNA can be used in applications including RT-PCR, Northern blotting, nuclease protection, and in vitro translation. Kit includes an on-column DNase treatment.

These kits eliminate phenol/choloroform extractions or time consuming steps, such as CsCl gradient ultracentrifugation and precipitation with isopropanol or LiCl.

Note, for the isolation of total RNA from whole blood and to attain higher RNA yields, use our Blood RNA Isolation Kits (#R6814 series), as they are specially designed for effective hemolysis and hemoglobin removal.

RNA Isolation and Purification

Manipulation of RNA is inherently more challenging than that of DNA since RNase is not only ubiquitous to most laboratories, it is also extremely unstable. Omega RNA Kits allow for reliable isolation of RNA from a variety of sources with its HiBind spin column method. These systems involve the simultaneous lysis of cells from a variety of sources of endogenous RNases. This is accomplished by using proprietary denaturing buffers that enhance the reversible binding of RNA to the HiBind silica matrix. While cells can be lysed directly, tissues are disrupted or homogenized using common laboratory supplies. Binding conditions are further adjusted and lysates are applied to the spin columns that selectively bind RNA molecules greater than 200 nt in length, thus favoring mRNA isolation. Successive washes with specially formulated buffers remove inhibitors and contaminants, allowing subsequent elution of pure RNA with water. As with other Omega products, phenol and chloroform extraction are eliminated. Kits are tailor made for specific cells and tissue sources to ensure consistent and reliable RNA preps, every time.

Storage and Stability - Kit isolation components are guaranteed for at least 12 months from the date of purchase, when stored as recommended in supplied instruction manuals.

V-Spin Columns are equipped with a standard outlet luer at its bottom for an easy to use and leak-free connection to vacuum manifolds or luer hub needles. V-Columns can be used with centrifugation and/or vacuum manifold protocols.

Overview

The E.Z.N.A.® Total RNA Kit I provides a straightforward and rapid method for the isolation of up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues. Multiple samples of up to 1 x 10⁷ eukaryotic cells or 30 mg of tissue can be processed in parallel in fewer than 20 minutes. Purified RNA can be used in many downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.

• Rapid – 20 minutes or less
• Safe – No phenol/chloroform extractions
• Versatile – Spin and vacuum formats available
• High quality – Purified RNA suitable for a variety of downstream applications

Specifications

For Research Use Only. Not for use in diagnostic procedures.