Omega Bio-Tek
- Compare with QIAGEN’s QIAamp® Viral RNA Kits -
#52904 & #52906 Mini-Kits; #965652 & #965662 Plate-Kits
Isolation of Viral RNA from Plasma, Serum, Urine, Cell Culture Supernants, and other Cell-Free Body Fluids.
Omega Biotek Viral RNA Mini Kits and EZ-96 plate Kits are for the isolation of viral RNA from cell-free fluids, such as plasma, serum, urine and cell culture supernatants. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient, and reliable. These kits have been tested for isolating viral nucleic acids from hepatitis A and C, as well as HIV. These kits are also suitable for the isolation of total RNA from cultured cells, tissues, and bacteria. The purified viral RNA is suitable for downstream applications, such as RT-PCR.
Ct values of recovered RNA isolation with the E.Z.N.A.® Viral RNA Kit. Hepatitis B virus was isolated using the E.Z.N.A.® Viral RNA Kit. A 10-fold dilution series of the recovered RNA was used in a SYBR® Green-based real-time PCR reaction. Each reaction was performed in triplicate.
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Overview: The Viral RNA Isolation Kits use the reversible binding properties of our proprietary silica-based HighBind matrix and spin technology. Combined with the speed of mini-spin columns and vacuum manifold technology, both our mini and 96-well plate kits can process multiple samples at the same time. The process is fast and simple. First, the sample is lysed under highly denaturing buffer conditions. This step inactivates the RNases and protects the intact Viral RNA from degradation. The buffer conditions are adjusteand then the samples are loaded into the HighBind RNA spin column or 96-well plate. After the samples undergo two washing steps, the purified viral RNA is eluted with RNase free water.
RNA Isolation and Purification
Manipulation of RNA is inherently more challenging than that of DNA since RNase is not only ubiquitous to most laboratories, it is also extremely unstable. Omega RNA EZ-Kits and EZ-96 plate Kits allow for reliable isolation of RNA from a variety of sources with its HighBind spin column method. These systems involve the simultaneous lysis of cells from a variety of sources of endogenous RNases. This is accomplished by using proprietary denaturing buffers that enhance the reversible binding of RNA to the HighBind silica matrix. While cells can be lysed directly, tissues are disrupted or homogenized using common laboratory supplies. Binding conditions are further adjusted and lysates are applied to the spin columns that selectively bind RNA molecules greater than 200 nt in length, thus favoring mRNA isolation. Successive washes with specially formulated buffers remove inhibitors and contaminants, allowing subsequent elution of pure RNA with water. As with other Omega products, phenol and chloroform extraction are eliminated. Kits are tailor made for specific cells and tissue sources to ensure consistent and reliable RNA preps, every time.