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AUTOMATION READY for ALL Popular Magnetic Processors

• Time Saving Accuracy: Prefilled plates reduce hands-on time. Reduction of user-associated errors
   Reduction of hands-on time with no reagent preparation (for bind, wash, and elute steps)
• Convenient: Includes all reagents and plasticware required.
   Inclusion of tip combs and other required plasticware in the kit for user convenience
• Automatable: Ready-to-load scripts available.  • Safe - No organic solvents
• Quality: Reproducible DNA extraction from a variety of samples.
   Purified DNA suitable for downstream applications such as next-generation sequencing, qPCR, PCR, and microarrays
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The Mag-Bind Blood & Tissue DNA HDQ Prefilled 96 Kit includes automation-ready, prefilled deep well plates in 96-well SBS format. The configuration of this kit works seamlessly on magnetic processors such as the Thermo Fisher Scientific KingFisher® Flex, QIAGEN BioSprint®, and Applied Biosystems MagMAX® Express-96. Included in this kit are all plastic accessories, including tip combs, that are needed for an extraction run. This kit offers a versatile method for isolating high-quality DNA from a wide variety of samples, including fresh or frozen animal cultured cells and tissues, buccal swabs, mouse tail snips, dried blood spots, up to 250 μL whole blood, and up to 500 μL saliva.

With no reagent set-up and the quick magnetic response of the Mag-Bind® Particles PF-HDQ magnetic beads, this kit reduces hands-on time, increases extraction accuracy, and reduces overall processing time. The purification procedure provides high-quality DNA suitable for direct use in most downstream applications, including amplification, next generation sequencing (NGS), and enzymatic reactions.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURESSPECIFICATIONS
Starting MaterialWhole blood, saliva, swabs, mouse tails, buffy coat, dried blood spots, cultured cells
Starting AmountVariable (depending on the sample input)
Elution Volume100 µL
Processing ModeAutomated, Post-lysis
Throughput4 x 96
Nucleic Acid Binding TechnologyMagnetic Beads
Run Time~40 minutes.
For scripting support, inquire for complete information.
Downstream Application(s)qPCR, NGS, microarray, etc
Storage ConditionsStore all components at room temperature in an upright position.
Store Proteinase K Solution at 2-8°C

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Protocols are available for the following automated platforms (INQUIRE):

•  Thermo Fisher Scientific KingFisher® Flex
•  QIAGEN BioSprint®
•  Applied Biosystems MagMAX® Express-96
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Table 1.
 The average DNA yield from 250 μL whole blood samples and 10 mg mouse kidney tissue samples were obtained using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit and eluted in 100 μL volume. DNA yield was determined using Thermo Scientific’s NanoDrop® 2000c. The absorbance ratios > 1.8 indicate high purity of the isolated DNA.

Figure 2. The size of genomic DNA purified from mouse kidney tissue samples using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit was analyzed on the Agilent TapeStation® 2200. The purified DNA is of high molecular weight, with the software analyzing it to be > 60 kb. The DIN values are all > 8.2, indicating highly intact DNA of superior quality.


Figure 3. Average Ct values obtained amplifying the purified DNA from blood and tissue samples using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit . The ΔCt value was ~3.3, indicating high-quality DNA that is free of inhibitors.

OMEGA Bio-tek Mag-Bind Blood & Tissue DNA HDQ Prefilled 96 Deep Well Plate Prep Kit, 4 x 96 preps/kit

Tissue, Blood & Cultured Cell Kit
Mag-Bind Blood & Tissue DNA HDQ, Prefilled 96 Deep Well Plate Kit, 4 x 96 preps/kit


AUTOMATION READY for ALL Popular Magnetic Processors

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SKU: M6399-01PF96
Old price: $1,685.60
$1,569.40