Table 1. The average DNA yield from 250 μL whole blood samples and 10 mg mouse kidney tissue samples were obtained using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit and eluted in 100 μL volume. DNA yield was determined using Thermo Scientific’s NanoDrop® 2000c. The absorbance ratios > 1.8 indicate high purity of the isolated DNA.
Figure 2. The size of genomic DNA purified from mouse kidney tissue samples using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit was analyzed on the Agilent TapeStation® 2200. The purified DNA is of high molecular weight, with the software analyzing it to be > 60 kb. The DIN values are all > 8.2, indicating highly intact DNA of superior quality.
Figure 3. Average Ct values obtained amplifying the purified DNA from blood and tissue samples using the Mag-Bind® Blood & Tissue DNA HDQ Prefilled 96 Kit . The ΔCt value was ~3.3, indicating high-quality DNA that is free of inhibitors.