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The Yeast DNA Mini EZ SpinKit combines the power of High Binding membrane technology with the time-tested consistency of alkaline SDS lysis  of yeast cells to yield high quality DNA in less than an hour.

The E.Z.N.A.® Yeast Plasmid Mini Kit combines the power of HiBind® technology with the alkaline-SDS lysis of yeast cells to deliver high-quality DNA in less than 1 hour. Omega Bio-tek’s HiBind® DNA Mini Columns facilitate the binding, washing, and elution steps, thus enabling multiple samples to be processed simultaneously. Yields will vary according to plasmid copy number, yeast strain, and growth conditions. Since yeast normally has very low plasmid copy number, the maximum yield from a 5 mL culture is ~ 1 μg.

The Yeast plasmid protocol has been used to successfully isolate autonomous plasmids from S.cerevisiae. Because its alkaline lysis procedure is modified, genomic DNA is virtually eliminated from the preparation. Further, the method can be easily adapted for plasmid isolation from E.coli.

Omega Plasmid DNA Isolation Kits from Life Science Products, Inc. combine the time tested alkaline-lysis and spin-column methodogy with our proprietary HiBind silica membrane-like matrix technology. By rapidly and quantitatively binding DNA, these Omega HiBind spin-columns effectively eliminate labor intensive steps required in traditional plasmid purification including phenol-chloroform extraction, cesium chloride banding, alcohol precipitation, and gravity-flow ion-exchange column procedures. In addition, this HighBind matrix eliminates common DNA shearing and silica resin carry-over often observed when compared to similar, competitive ‘bind-wash-elute’ type spin-columns. Up to 90% recovery of pure plasmid DNA from fragments; yields vary according to plasmid copy number, E.coli strain, and conditions of growth.

Applications: Plasmid DNA Isolation Kits are used for screening, restriction endonuclease digestions, manual or automated fluorescent DNA sequencing, transfection, ligations, and other manipulations.

Storage and Stability - LSPI’s Omega Plasmid Kit isolation components are guaranteed for at least 12 months from the date of purchase, when stored as recommended in supplied instruction manuals.