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Description 

The PM5100 3-color Pre-Stained Protein Ladder High Range is a ready-to-use three-color protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color Pre-Stained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. 

 

Features

  • Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.

  • Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)

 

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

 

Quality Control 

Under suggested conditions, PM5100 ExcelBand™ 3-color Pre-Stained Protein Ladder High Range resolves 14 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for 24 months 

PM5100-2, MAXI-SIZE (6x250µl) ExcèlBand™ - 3 Color, 14 Pre-Stained Protèin Laddèr, High Range (9-245 kDa)

MAXI-SIZE (6 x 250µl) 1500µl per unit
SMOBIO ExcelBand™
3 color 14 Pre-Stained Protein Ladder, High Range
10 to 245 kDa in Tris-Glycine Buffer, 9 to 235 kDa in Bis-Tris
(MOPS) buffer, and 10 to 235 kDa in Bis-Tris (MES) buffer
SKU: PM5100-2
$414.70