ExcelRT™ Reverse Transcriptase 
200 Units/µl, (10,000 Units x 2) 20,000 Units 

Description 
The ExcelRT Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, a RNA dependent DNA polymerase capable of generating the first strand cDNA using RNA template. 

The FluoroStain Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.

Features
• High yield
• Thermostable, up to 50°C, during first strand synthesis
• High processivity, generating cDNA up to 8 kb
• Reduced RNase H ribonuclease activity
• No detectable a 3’→ 5’ exonucleolytic proofreading function
• Thermal stable for at least 4 weeks when stored at 4°C

Content Components - Volume

Fig.1.)  ExcelRT Reverse Transcriptase can synthesize 1st strand cDNA using only 5 fg of total RNA in solution. The bands in the gel represent the results of GAPDH (230 bp) from two step RT-PCR reaction. (M: DM2100)
Lane 1: 500 pg 　
Lane 2: 50 pg 　
Lane 3: 5 pg 　
Lane 4: 500 fg
Lane 5: 50 fg
Lane 6: 5 fg 
Lane 7: NTC 

5X reaction buffer
250 mM Tris-HCl (pH 8.3), 50mM DTT, 375 mM KCl and 15 mM MgCl2

Storage buffer
20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol 

Unit definition
One unit is defined as the amount of enzyme that will incorporate 1 nM of dTTP into acid-insoluble material in 10 minutes at 37°C using Poly(A)•oligo(dT)₂₅ as template-primer. 

Storage
-20°C ≥ 24 months