Fig.1.) ExcelRT Reverse Transcriptase can synthesize 1st strand cDNA using only 5 fg of total RNA in solution. The bands in the gel represent the results of GAPDH (230 bp) from two step RT-PCR reaction. (M: DM2100)
Lane 1: 500 pg
Lane 2: 50 pg
Lane 3: 5 pg
Lane 4: 500 fg
Lane 5: 50 fg
Lane 6: 5 fg
Lane 7: NTC
5X reaction buffer250 mM Tris-HCl (pH 8.3), 50mM DTT, 375 mM KCl and 15 mM MgCl2
Storage buffer20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
Unit definitionOne unit is defined as the amount of enzyme that will incorporate 1 nM of dTTP into acid-insoluble material in 10 minutes at 37°C using Poly(A)•oligo(dT)
25 as template-primer.
Storage-20°C ≥ 24 months